Fig 2: Effects of PAMPs and HIV-1 on the level of TLR expression in cell cultures. (A) qRT-PCR of TLR10, TLR2, and TLR1 mRNA from MCF-10A cells treated with TLR2 PAMP Pam3CSK4, TLR4 PAMP LPS, TLR7/TLR8 PAMP ssRNA40, and cell-free HIV-1 BAL. (B) q-RT-PCR in THP-1 cells. (C) qRT-PCR in primary BM cells. Data set is representative of three different experiments completed in triplicate. Statistic marks: *p < 0.05, **p < 0.01 for the Mann-Whitney t-tests, each group compared to medium-treated group (Medium).

Fig 3: The four CD8+ T cell-related genes were upregulated in cervical cancer and associated with prognosis. (A) The heatmap of the relative levels of IGSF6, TLR10, FCRL3, and IFI30 in normal and cervical cancer tissues. (B) The correlations of IGSF6, TLR10, FCRL3, IFI30, and CD8+ T cell infiltration. The overall survival curves of (C) IGSF6, (D) TLR10, (E) FCRL3, and (F) IFI30 in cervical cancer.

Fig 4: Validation of immune cell infiltrations and gene profile in the validation cohort. (A) The heatmap of the relative levels of IGSF6, TLR10, FCRL3, and IFI30 in normal and cervical cancer tissues in the validation cohort. (B) The relative levels of immune cell infiltrations in normal and cervical cancer groups in the validation cohort. (C) The correlations of IGSF6, TLR10, FCRL3, IFI30, and CD8+ T cell infiltration in the validation cohort. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Fig 5: Significantly elevated expression level of TLR1 and TLR10 in HIV-1 infected human primary BM cells. Expression of TLR1 and TLR10 as measured by qRT-PCR with the mRNA extracted from primary BM cells collected from HIV-1 negative Hamilton, Canada women (HIV-N Hamilton) and Nigerian HIV-1 negative (HIV-N) and HIV-1 positive (HIV-P) women. TLR1 expression is shown on right (p = 0.0006) whereas TLR10 expression is shown on left (p < 0.0001).